重慶市華雅干細胞技術有限公司 ，  ：

華雅集團旗下授權代理TTAKARA Cellartis干細胞產品

圖片為展示效果。實際產品為細胞株+培養基套裝

人iPS誘導多能干細胞

(Human Induced Pluripotent Stem Cells)

產品英文名稱：Cellartis® Human iPS Cell Lines

產品中文名稱：Cellartis® 人iPS誘導多能干細胞

Cellartis人iPS細胞（Human iPS Cell Line）產品是達到了工業客戶使用標準生產的干細胞產品。該產品包括細胞和用于增殖培養的Cellartis DEF-CS Culture System培養體系。iPS細胞已經在制品化時完成了對DEF-CS培養體系的適應。Cellartis DEF-CS Culture System培養體系是組分明確而且不依賴飼養層的便捷的干細胞培養體系。Cellartis DEF-CS Culture System適合高效率地增殖培養干細胞，并且保持干細胞在增殖過程中幾乎無分化地生長，這使得不需要在大規模增殖培養過程中進行細胞篩選。因此，Cellartis人iPS細胞（Human iPS Cell Line）產品是一套完整的產品體系，對于科研級別和工業規模的需求都適合。

Cellartis人iPS誘導多能干細胞特點

☆細胞系通過使用缺陷型多順反子逆轉錄病毒（defective polycistronic retrovirus）技術重編程人皮膚成纖維細胞以轉入Oct-4，SOX2，KLF4和c-Myc

☆細胞系的純度和干細胞特征經過廣泛分析，包括解凍后的恢復，支原體和細菌檢測，干細胞特異性標記物的表達（Oct-4，NANOG，SSEA-3，SSEA-4，TRA-1 -60，TRA-1-81），不表達分化標記（β-3微管蛋白，FOXA2，ASMA）和預期核型

☆用Cellartis DEF-CS培養系統優化培養，試劑盒包括Cellartis DEF-CS 100培養系統

☆適用于所有主要應用; 細胞在分化時可以分化形成所有三個胚層的衍生物

Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc

Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected Karyotype

Optimized for culture with the Cellartis DEF-CS Culture System

Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated

Kits include the Cellartis DEF-CS 100 Culture System

Cellartis 人iPS誘導多能干細胞參數

不同貨號對應供體年齡性別冷凍代數和已確認分化能力

iPS誘導多能干細胞的應用

細胞治療、藥物監測和疾病模型

Cellartis 人iPS誘導多能干細胞組份

不同供體的iPS細胞系和DEF-CS 100*培養基

*培養基包含基礎培養基、添加劑和包被劑。

保存：細胞 ≤ –150°C，添加劑 –20°C,基礎培養基和包被劑4 °C。

A. Cellartis Human iPS Cell Lines

Cellartis human iPS cell line P11025 (Cat. No. Y00180, not sold separay; sold as a part of Cat.No. Y00185)

Cellartis human iPS cell line P11032 (Cat. No. Y00220, not sold separay; sold as a part of Cat.No. Y00225)

Cellartis human iPS cell line 4 (ChiPSC4) (Cat. No. Y00260, not sold separay; sold as a part of Cat. No. Y00265)

Cellartis human iPS cell line 7 (ChiPSC7) (Cat. No. Y00270, not sold separay; sold as a part of Cat. No. Y00275)

Cellartis human iPS cell line 12 (ChiPSC12) (Cat. No. Y00280, not sold separay; sold as a part of Cat. No. Y00285)

Cellartis human iPS cell line 18 (ChiPSC18) (Cat. No. Y00300, not sold separay; sold as a part of Cat. No. Y00305)

Cellartis human iPS cell line 21 (ChiPSC21) (Cat. No. Y00310, not sold separay; sold as a part of Cat. No. Y00315)

Cellartis human iPS cell line 22 (ChiPSC22) (Cat. No. Y00320, not sold separay; sold as a part of Cat. No. Y00325)

B. Cellartis DEF-CS 100 Culture System

· Cellartis DEF-CS 100 Culture System (Cat. No. Y30020, not sold separay; sold as a part of Cat.Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00305, and Y00315. A larger 500ml version of this product is sold as Cat. No. Y00310.)

o DEF-CS Basal Medium (100 ml)

o DEF-CS COAT-1 (for 100 ml) (800 µl)

o DEF-CS GF-1 (for 100 ml) (300 µl)

o DEF-CS GF-2 (for 100 ml) (100 µl)

o DEF-CS GF-3 (for 100 ml) (40 µl)

另行準備材料：PBS+TrypLE 選擇酶+TC處理ps表面培養器皿

The following materials are required but not supplied:

· PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)

· PBS Dulbecco's without Ca2+ or Mg2+ (D-PBS –/–)

· TrypLE Select Enzyme (1X), no phenol red

· Cell culture vessels, Tissue culture treated polystyrene surface

· General cell culture equipment used in cell culture laboratory

參考文獻：

1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426.

2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190.

3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4.

4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319.

Cellartis 人iPS誘導多能干細胞訂購信息

不同貨號的區別：iPS細胞供體不同

 ?華雅集團旗下授權代理TTAKARA Cellartis干細胞產品，Cellartis的多能細胞培養系統包括iPS誘導多能干細胞株、iPS誘導多能干細胞和ES胚胎干細胞培養基，iPS基因編輯用單細胞克隆培養基和多能細胞檢測用單克隆抗體。

重慶市華雅干細胞技術有限公司以“專注干細胞研究，提升干細胞品質”為發展理念，專業從事干細胞基礎研究、干細胞再生醫學臨床應用研究及干細胞技術服務，自主研發產品熱賣中：人間充質干細胞無血清培養基 

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