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簡(jiǎn)要描述:hES-MP 002.5成骨細(xì)胞礦化人間充質(zhì)前體細(xì)胞是從人胚胎干細(xì)胞誘導(dǎo)分化而得到的間充質(zhì)干細(xì)胞前體細(xì)胞(multipotent mesenchymal progenitors)。均質(zhì)的,批次檢測(cè)的細(xì)胞群; 沒(méi)有供體到供體的可變問(wèn)題細(xì)胞強(qiáng)壯易培養(yǎng)與成人人類(lèi)間充質(zhì)干細(xì)胞高度相似能夠分化成中胚層譜系擴(kuò)增后高礦化潛力
品牌 | 其他品牌 | 貨號(hào) | Y10090 |
---|---|---|---|
規(guī)格 | 1 mL | 供貨周期 | 一個(gè)月 |
主要用途 | 成骨分化用間充質(zhì)細(xì)胞前體 |
重慶市華雅干細(xì)胞技術(shù)有限公司 , :
華雅集團(tuán)旗下授權(quán)代理TTAKARA Cellartis干細(xì)胞產(chǎn)品
圖片為展示效果,實(shí)際產(chǎn)品為hES-MP 002.5細(xì)胞株
高礦化潛力的間充質(zhì)前體細(xì)胞
產(chǎn)品英文名稱(chēng):hES-MP 002.5,Human Multipotent Mesenchymal Progenitor Cells
產(chǎn)品中文名稱(chēng):人類(lèi)胚胎干細(xì)胞來(lái)源的間充質(zhì)前體細(xì)胞
hES-MP 002.5是從人胚胎干細(xì)胞誘導(dǎo)分化而得到的間充質(zhì)干細(xì)胞前體細(xì)胞(multipotent mesenchymal progenitors)。hES-MP 002.5與人間充質(zhì)干細(xì)胞(human mesenchymal stem, MS)高度相似,可以分化成為多種中胚層細(xì)胞種類(lèi)。hES-MP 002.5由人胚胎干細(xì)胞系SA002.5 ES(a subclone of SA002)分化得來(lái),可以向骨細(xì)胞進(jìn)行高效率的分化,分化過(guò)程具有著高級(jí)別的礦物化潛力(mineralization potential)。hES-MP 002.5細(xì)胞產(chǎn)品是高度均勻的、高潛力的間充質(zhì)干細(xì)胞前體細(xì)胞,適合于科研級(jí)別以及工業(yè)規(guī)模的間充質(zhì)干細(xì)胞生產(chǎn)。
hES-MP 002.5 cells are human embryonic stem (ES) cell-derived multipotent mesenchymal progenitors that resemble adult human mesenchymal stem (MS) cells and can be differentiated into several mesodermal lineages. The cells are derived from the SA002.5 ES cell line, a subclone of SA002, known for efficient osteogenic lineage differentiation and high mineralization potential with extended culture. hES-MP 002.5 cells are a homogeneous, robust source of mesenchymal progenitors, ideal for bulk production of mesenchymal cells.
均質(zhì)的,批次檢測(cè)的細(xì)胞群; 沒(méi)有供體到供體的可變問(wèn)題
細(xì)胞強(qiáng)壯易培養(yǎng)
與成人人類(lèi)間充質(zhì)干細(xì)胞高度相似
能夠分化成中胚層譜系
擴(kuò)增后高礦化潛力High mineralization potential with extended expansion
間充質(zhì)誘導(dǎo)和分化研究
修復(fù)和再生的組織工程模型
Mesenchymal induction and differentiation studies
Tissue engineering models of repair and regeneration
2017應(yīng)用實(shí)例:
研究人員使用Cellartis® hES-MP 002.5細(xì)胞表明了流動(dòng)體可以刺激間充質(zhì)干細(xì)胞的骨基質(zhì)產(chǎn)生。
A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds
Sasima Puwanun Robin M. Delaine‐Smith Helen E. Colley Julian M. Yates Sheila MacNeil Gwendolen C. Reilly
Journal of Tissue Engineering and Regenerative Medicine,First published: 9 May 2017 https://doi.org/10.1002/term.2462
組份
1瓶細(xì)胞
1 frozen vial of Cellartis® hES-MP 002.5
保存:≤ -150°C。
使用方法
Unpacking of Cellartis® hES-MP 002.5
NOTE! For your protection: Wear a protective face mask and protective gloves. Use forceps when
handling a frozen vial. Never hold the vial in your hand as the cryo vial may explode due to rapid
temperature changes.
NOTE! When transferring the cells from the transport vessel to long time storage, immediate transfer is essential since variations in temperature may have an adverse effect on cell survival and quality.
1. Unpack Cellartis® hES-MP 002.5 as soon as possible after arrival.
2. Check all materials for leakage or breakage.
3. Using forceps immediay place the vial in liquid nitrogen at ≤ -150°C.
自備試劑:
產(chǎn)品 建議的制造商 貨號(hào)
basic Fibroblast Growth Factor (bFGF) Peprotech 100-18B
Dulbecco’s Modified Eagle Medium (DMEM) Gibco / Life Technologies 31966-021
胎牛血清(FBS) Gibco / Life Technologies 16140
明膠 Sigma-Aldrich G1890
PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS (-/-)) Gibco / Life Technologies 14190
青霉素/鏈霉素(PEST) Gibco / Life Technologies 15140-122
TrypLE Select™ Gibco / Life Technologies 12563-011
Cellartis hES-MP 002.5細(xì)胞傳30代,細(xì)胞群倍加數(shù)(cell population doubling level,縮寫(xiě)PDL)沒(méi)有下降。
Population doubling of human mesenchymal stem cells (hMSCs) and Cellartis hES-MP 002.5 cells through 30 passages. Unlike hMSCs, hES-MP 00.25 cells show no decrease in population doubling level.
品牌 | 貨號(hào) | 產(chǎn)品描述 | 規(guī)格 |
Cellartis | Y10090 | hES-MP 002.5 Human Multipotent Mesenchymal Progenitor Cells 人類(lèi)胚胎干細(xì)胞來(lái)源的間充質(zhì)前體細(xì)胞 | 1 mL |
?華雅集團(tuán)旗下授權(quán)代理TTAKARA Cellartis干細(xì)胞產(chǎn)品,Cellartis的多能細(xì)胞培養(yǎng)系統(tǒng)包括iPS誘導(dǎo)多能干細(xì)胞株、iPS誘導(dǎo)多能干細(xì)胞和ES胚胎干細(xì)胞培養(yǎng)基,iPS基因編輯用單細(xì)胞克隆培養(yǎng)基和多能細(xì)胞檢測(cè)用單克隆抗體。
重慶市華雅干細(xì)胞技術(shù)有限公司以“專(zhuān)注干細(xì)胞研究,提升干細(xì)胞品質(zhì)”為發(fā)展理念,專(zhuān)業(yè)從事干細(xì)胞基礎(chǔ)研究、干細(xì)胞再生醫(yī)學(xué)臨床應(yīng)用研究及干細(xì)胞技術(shù)服務(wù),自主研發(fā)產(chǎn)品熱賣(mài)中:人間充質(zhì)干細(xì)胞無(wú)血清培養(yǎng)基
咨詢Cellartis-Y10090 Human Multipotent Mesenchymal Progenitor cells , :
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